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Part:BBa_I716318:Design

Designed by: Hannah Cole   Group: iGEM07_Berkeley_UC   (2007-07-12)

Myoglobin attached to pBca 9145-1144 vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 337
    Illegal XbaI site found at 373
    Illegal XbaI site found at 426
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 337
    Illegal PstI site found at 519
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 337
    Illegal BglII site found at 16
    Illegal BamHI site found at 496
    Illegal XhoI site found at 507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 337
    Illegal XbaI site found at 373
    Illegal XbaI site found at 426
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 337
    Illegal XbaI site found at 373
    Illegal XbaI site found at 426
    Illegal PstI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1606


Design Notes

There are three internal restriction sites: EcoRI BglII and XhoI. For this part I removed BglII and XhoI.

Primers: Outside Forward cgttaAGATCTATGGTTCTGTCTGAAGGTGAATGG. Outside Reverse: ggtcatCTCGAGgttacGGATCCTCATCCCGAGCCACCCTGGTAACCC BglII Forward: GAAAGCTTCTGAAGACCTGAAAAAACATGGTGTTAC BglII Reverse: GTAACACCATGTTTTTTCAGGTCTTCAGAAGCTTTC XhoI Forward:GCTATGAACAAAGCTCTAGAGCTGTTCCGTAAAGA and XhoI Reverse: TCTTTACGGAACAGCTCTAGAGCTTTGTTCATAGC


Source

PBond Vector W6 APS-Myoglobin His6-tct and pBca 9145-1144

References